BBa_I20247

BBa_I20247 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I20247

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jason Kelly, Karen Wong, Nishant Bhat

Date created: 2007-08-14 11:00:00

Date modified: 2015-08-31 04:07:39

A device used to characterize promoters throught measurement of RFP expression

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_I20247_sequence (Version 1)

Description

Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing.

Notes

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Source

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Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
EcoR1-Not1-Xba1	1,21	feature/BioBrick, engineered_region
BBa_B0032	1,13	engineered_region, feature/BioBrick
RBS	7,10	ribosome_entry_site, feature/rbs
mrfp1	20,694	CDS, feature/cds
BBa_E1010	20,700	feature/BioBrick, engineered_region
barcode	701,725	feature/BioBrick, engineered_region
BBa_B0010	734,813	feature/BioBrick, engineered_region
stem_loop	745,788	feature/stem_loop, stem_loop
BBa_B0012	822,862	feature/BioBrick, engineered_region
T7 TE	829,848	stem_loop, feature/stem_loop
polya	849,862	polyA_site, feature/polya
stop	855,855	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I20247/1

Member of these Collections

• iGEM Parts Registry