Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Kelly, Karen Wong, Nishant Bhat
Date created: 2007-08-14 11:00:00
Date modified: 2015-08-31 04:07:39
A device used to characterize promoters throught measurement of RFP expression
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_I20247_sequence (Version 1) |
Description
Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing.Notes
.Source
.| Sequence Annotation | Location | Component / Role(s) |
| EcoR1-Not1-Xba1 BBa_B0032 RBS mrfp1 BBa_E1010 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,21 1,13 7,10 20,694 20,700 701,725 734,813 745,788 822,862 829,848 849,862 855,855 | feature/BioBrick engineered_region engineered_region feature/BioBrick ribosome_entry_site feature/rbs CDS feature/cds feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop |