Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jerome Bonnet
Date created: 2009-07-09 11:00:00
Date modified: 2015-08-31 04:07:40
Bacteriophage Bxb1 Integrase-Freiburg standard
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_I20284_sequence (Version 1) |
Description
Bxb1 integrase is a recombinase enzyme from the serine recombinase family. Bxb1 naturally catalyze integration of the phage genome into the GroEL1 gene of M.segmentis, which contains the attB site [2]. Minimal attB, attP,[2],[3] attL and attR [4] have been characterized. Excisionase has been identified, is relatively big (28kDa) for a RDF, and is efficient both in vivo and in vitro [4]. In vitro recombination is very efficient ranging a 100% efficiency in 1hour for integration [2] and for excision [4]. Interestingly the excisionase subunit does not bind DNA and acts stoechiometrically on Bxb1 integrase [4]. The enzyme does not require supercoiled DNA, and can also works with linear products [2]. The enzyme also works in human, rat, and mouse cells [5, 6].When recombination sites are placed in the same DNA molecule, recombination outcome is inversion of the sequence they surround.
Notes
Sequences cleaned up for restriction sites according to Freiburg standard (25)Source
Source: http://www.uniprot.org/uniprot/Q9B086References:
[1] Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671.
[2] Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene. Kim AI, Ghosh P, Aaron MA, Bibb LA, Jain S, Hatfull GF. Mol Microbiol. 2003
[3] The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Ghosh P, Kim AI, Hatfull GF. Mol Cell. 2003 PMID:14636570
[4] Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562.
[5] Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393
[6] A diversity of serine phage integrases mediate site-specific recombination in mammalian cells. Keravala A, Groth AC, Jarrahian S, Thyagarajan B, Hoyt JJ, Kirby PJ, Calos MP. Mol Genet Genomics, 2006 PMID: 16699779