| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_I20285_sequence (Version 1)
|
Description
This protein is a recombination directionality factor (RDF). Association with Bxb1 integrase, and triggers recombination between AttL and attR and excision of the phage genome. Bxb1 Excisionase is relatively big (28kDa) for a RDF, and is efficient both in vivo and in vitro [4]. In vitro recombination is very efficient ranging a 100% efficiency in 1hour for integration [2] and for excision [4]. Interestingly the excisionase subunit does not bind DNA and acts stoechiometrically on Bxb1 integrase [4]. Reverse inversion reaction when attL and attR in same DNA molecule. No known catalytic activity per se.
Notes
sequence cleaned for restriction sites according to Freiburg standard.
Source
http://www.uniprot.org/uniprot/Q9B074
