BBa_I20299

BBa_I20299 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I20299
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Francois St-Pierre
Date created: 2010-06-20 11:00:00
Date modified: 2015-08-31 04:07:40

RBS from T7 gene 4, optimal aligned spacing



    • Details

    Types
    DnaRegion

    Roles
    ribosome_entry_site

    RBS

    Sequences BBa_I20299_sequence (Version 1)

    Description

    RBS derived from phage T7 gene 4. Similar to I20298, but with the last (3') nucleotides removed. This produces an optimal aligned spacing of 5 nucleotides between the Shine-Dalgarno (SD) sequence and the ATG of the following CDS, when the RBS is placed immediately before the ATG i.e. without Biobrick "scar". For information on "optimal aligned spacing", see Chen et al., (1994) NAR, 22(23):4953-7 [PMID 7528374]

    Notes

    See "long description"

    Source

    Derived from the RBS of phage T7 gene 4

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I20299/1
     

    Attachments

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