Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jerome Bonnet
Date created: 2012-04-02 11:00:00
Date modified: 2015-08-31 04:07:41
Bxb1 BP Data Register
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_I20532_sequence (Version 1) |
Description
The DNA data register in BP and LR states consist of a constitutive promoter (BBa_J23119) flanked by BP or LR recombination sites positioned in opposite orientation, resulting in DNA inversion when recombined. A Rrnp T1 terminator (BBa_J61048) was added in reverse orientation upstream of the promoter to prevent transcriptional read-through in the opposite orientation, so that in each state, only one fluorescent protein is visibly expressed. On each side of the recombination target, we cloned superfolder GFP and mKate2 under translational control of measured strong RBSs (BIOFAB pilot C-dog project http://biofab.org/data).Notes
Recombination sites can have some terminator activity-Strong RBS preferabe at low copy numberSource
Multiple| Sequence Annotation | Location | Component / Role(s) |
| mKate2 Invitrogen RBS AttB Bxb1 J61048 terminator J23119 JBEI Superfolder GFP B0015 attP Bxb1 | 1,696 697,726 727,776 830,942 1035,1069 1267,1300 1301,2020 2021,2149 1211,1263 | feature/protein CDS feature/rbs ribosome_entry_site sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter ribosome_entry_site feature/rbs feature/protein CDS feature/misc sequence_feature feature/misc sequence_feature |