F plasmid

BBa_I50000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I50000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Reshma Shetty

Date created: 2005-11-16 12:00:00

Date modified: 2015-08-31 04:07:41

F plasmid backbone with BioBrick sites removed.

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_I50000_sequence (Version 1)

Description

This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
sopC(IncD) repeat region	3938,4640	sequence_feature, feature/misc
parB	2893,3864	feature/cds, CDS
parA	1718,2893	CDS, feature/cds
sequence derived from F plasmid in GenBank Accession Number AP001918	1,1390	feature/misc, sequence_feature
repE	384,1139	CDS, feature/cds
ori2 (oriS) single copy replication origin	1,290	feature/misc, sequence_feature
C to T mutation (eliminates PstI site)	2553,2553	feature/s_mutation, point_mutation
A to T mutation (eliminates SpeI site)	3711,3711	feature/s_mutation, point_mutation
C to T mutation (eliminates PstI site)	1013,1013	point_mutation, feature/s_mutation
C to G mutation (eliminates XbaI site, noncoding region)	183,183	sequence_alteration, feature/mutation
DnaA box	89,97	operator, feature/operator
A/T region	101,147	feature/misc, sequence_feature
iteron (repeat region)	192,211	feature/misc, sequence_feature
iteron (repeat region)	213,232	sequence_feature, feature/misc
iteron (repeat region)	246,265	feature/misc, sequence_feature
iteron (repeat region)	268,287	sequence_feature, feature/misc
DnaA box	81,88	feature/operator, operator
sequence derived from F plasmid in GenBank Accession Number AP001918	1720,4640	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I50000/1

Member of these Collections

• //classic/plasmid/uncategorized
• //plasmid/component/origin
• iGEM Parts Registry