Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2005-12-03 12:00:00
Date modified: 2015-08-31 04:07:41
oriV origin
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_I50010_sequence (Version 1) |
Description
oriV origin which requires the presence of the RK2-encoded TrfA replication protein (resident on the genome of a host-strain) to be functional. Vector copy number will be 20-50 if this replication origin is active. For this origin to be functional, the strain background must contain the trfA gene. Generally the appropriate strain has a copy-up TrfA mutant protein controlled by the ParaBAD promoter. Thus, L-arabinose can be added to the media to induce expression of the TrfA protein and enable replication at the oriV origin. The origin will not be functional in host strains without this protein. Generally, this origin is used as a secondary, inducible copy number origin in F plasmid based vectors. It allows conditional amplication of the vector copy number when in the appropriate host strain.Similar to part of the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site, a single copy origin like BBa_I50000) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering.
| Sequence Annotation | Location | Component / Role(s) |
| iteron (repeat unit) iteron (repeat unit) iteron (repeat unit) iteron (repeat unit) iteron? (repeat unit) iteron? (repeat unit) iteron? (repeat unit) iteron? (repeat unit) | 199,216 244,261 461,477 406,420 266,283 222,238 439,456 178,193 | sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc |