Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2006-01-09 12:00:00
Date modified: 2015-08-31 04:07:41
Minimal pUC19-derived high copy replication origin
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_I50020_sequence (Version 1) |
Description
High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites.| Sequence Annotation | Location | Component / Role(s) |
| rep (pMB1) ORI origin of replication RNAII transcript RNAI transcript promoter -35 RNAI transcript promoter -10 RNAI transcript RNAII transcript promoter -10 sequence RNAII transcript promoter -35 sequence G to A, nonfunctional mutation G to A, nonfunctional mutation G to A, nonfunctional mutation C to T, nonfunctional mutation C to T, nonfunctional mutation | 16,630 615,615 27,615 63,615 204,209 182,187 66,173 48,53 27,32 261,261 265,265 274,274 303,303 467,467 | feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter promoter feature/promoter sequence_feature feature/misc feature/promoter promoter feature/promoter promoter feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration |