Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2010-06-20 11:00:00
Date modified: 2015-08-31 04:07:42
pSC101 temperature sensitive origin of replication
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_I50052_sequence (Version 1) |
Description
BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector.Notes
See design notes for [[Part:BBa_I50042]].Source
This part is derived from BBa_I50042. It contains a point mutation that changes Alanine to Valine in RepA protein.| Sequence Annotation | Location | Component / Role(s) |
| Terminator par region inverted repeat Tn3 integration site inverted repeat inverted repeat inverted repeat AT rich region direct repeat direct repeat direct repeat mutated SpeI site -35 inverted repeat promoter region -10 inverted repeat RBS RBS repA inverted repeat GCT->GTT ts mutation (Ala->Val) G to A difference with canonical pSC101 vector sequence putative polyA C to T difference with canonical pSC101 vector sequence G to C difference with canonical pSC101 vector sequence T to C difference with canonical pSC101 vector sequence | 46,75 137,504 139,152 158,162 165,181 192,215 221,238 635,717 716,735 737,756 769,788 814,819 828,833 838,859 846,865 850,855 862,884 870,882 879,884 886,1836 1847,1861 1051,1053 1655,1655 1947,1962 1750,1750 1912,1912 2002,2002 | feature/stem_loop stem_loop feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/mutation sequence_alteration feature/promoter promoter feature/misc sequence_feature feature/promoter promoter promoter feature/promoter sequence_feature feature/misc feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs feature/cds CDS feature/misc sequence_feature feature/mutation sequence_alteration sequence_alteration feature/mutation polyA_site feature/polya sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation |