Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rok Gaber
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:07:46
TEV protease (C-terminal half)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_I712078_sequence (Version 1) |
Description
C-terminal half of TEV (Tobacco Etch Virus) protease.TEV protease is a highly site-specific protease that is found in the Tobacco Etch Virus (TEV). One of the main uses of this protease is for removing affinity tags from purified proteins. But scientists developed assay for detecting transmembrane protein interactions on its base.
TEV protease is splitted into halves (N-terminal and C-terminal). These halves are inactive but assembles and regain activity if they are fused with interacting transmembrane proteins. For detecting TEV protease activity another fusion protein is constructed which is composed of transmembrane region, specific TEV protease cleavage site and kind of transcription factor. When both halves of TEV protease assembles and therefore split protease regains activity it cleaves third protein and transcription factor is released from membrane. Transcription factor is later translocated to the nucleus where induces transcription of promoter with reporter gene.
This is C-terminal half of this protease.