Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rok Gaber
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:07:46
CMV promoter - Myc tag - CCR5 (shortened) - TEV N
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_I712079_sequence (Version 1) |
Description
Human CCR5 shortened receptor fused with N-terminal half of TEV protease and Myc tag. This construct is under control of CMV promoter.Human CCR5 transmembrane receptor is a chemokine receptor which plays cruical role in HIV infection. This version of CCR5 is shortened for 50 AK from C-terminus, therefore is without cytosolic domain. Myc tag is fused to the N-terminal of this protein.
CCR5 (shortened) is also fused with N-terminal half of TEV protease. This construct is coexpressed with CD4 receptor fused with C-terminal half of TEV protease (in mammalian cells). When that kind of human cells are infected with HIV virus both halves of TEV protease assemble and split protein regains its function. This principle can be used for fighting HIV infection.
Source
Cytomegalovirus, Tobacco Etch Virus, Human| Access | Instance | Definition |
| public public | BBa_I712076 BBa_I712077 | BBa_I712076 TEV |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712076 BBa_I712077 | 1,1608 1617,1973 | BBa_I712076 TEV |