BBa_I713025

BBa_I713025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I713025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhenyu Shi

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:07:47

pEB-lambda

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_I713025_sequence (Version 1)

Description

pEASY-B blue/white screen vector with designed FRT flaked insert to block the lacZ activity.

When FLP is applied, the FRT insert will be deleted and the vector will recover the lacZ activity.

Notes

Use overlap-extension PCR to clone two parts of the pKD13.

Source

pKD13
pEASY-B

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacZ	1333,1490	feature/cds, CDS
FRT insert	366,1204	sequence_feature, feature/misc
kan(r)	2263,3057	feature/cds, CDS
amp(r)	3075,3935	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I713025/1

Member of these Collections

• iGEM Parts Registry