Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chunbo Lou
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:07:47
Riboregulator key 1
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_I714036_sequence (Version 1) |
Description
The same as J01008Notes
The Isaacs key1 riboregulator taR12 is modeled on the hok/sok post-segregational killing method of plasmid R1. It makes 25 base pairs with its counterpart, the riboregulator J01010 (aka "lock 1" or "crR12").Isaacs documentation (see reference #1):
Upon induction of the araC promoter (using 0.25% arabinose solution), the taR12 key activated the corresponding crR12 lock to express the downstream indicator, GFP, to levels of fluorescence 19-fold greater than without taR12 expression. Maximal expression was reached at 70 minutes after addition of arabinose solution, and was maintained after that point.