| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_I715000_sequence (Version 1)
|
Description
The amino half of the Cre recombinase gene (BBa_J61047) was cloned into plasmid pSB1A2. This half contains the first 570 base pairs in the gene. The carboxyl half (BBa_I7165) of the Cre recombinase gene was also cloned into plasmid pSB1A2 and contains the remaining 462 base pairs of the gene. Our eventual goal was to insert a hixC site in the middle of the gene while maintaining Cre recombinase's functionality. The location of our split site was chosen based off of outside literature in which Cre Recombinase had been successfully split as well as the protein structure of Cre recombinase.
Notes
None
Source
BBa_J61047
