| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_I715001_sequence (Version 1)
|
Description
The carboxyl half of the Cre recombinase gene (BBa_J61047) was cloned into plasmid pSB1A2. This half contains the last 462 base pairs in the gene. The amino half (BBa_I716500) of the Cre recombinase gene was also cloned into plasmid pSB1A2 and contains the first 570 base pairs of the gene. Our eventual goal is to insert a hixC site in the middle of the gene while maintaining Cre recombinase's functionality. The location of our split site was chosen based off of outside literature that demonstrated the successful splitting of Cre Recombinase, as well as the protein structure of Cre recombinase.
Notes
None
Source
BBa_J61047
