Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Waters
Date created: 2007-07-02 11:00:00
Date modified: 2015-08-31 04:07:49
hybrid pLac with UV5 mutation
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_I715003_sequence (Version 1) |
Description
This promoter sequence is a derivative of part BBa_R0011 which is a -35 and -10 recognition site with two lac operator 1's inserted in the spacer region of the RNAP recognition region and before the -35 RANP recognition region. This part utilizes the UV5 double mutation at the -10 RNAP recognition site to recruit RNAP without the help of a CAP binding region.Notes
The length of the fragment makes it cumbersome to gel purifySource
E.Coli genome| Sequence Annotation | Location | Component / Role(s) |
| -35 RNAP binding lac 01 lac 01 -10 RNAP binding UV5 -10 RNAP recognition region double mutation | 20,25 3,19 26,42 43,47 43,47 | promoter feature/promoter operator feature/operator operator feature/operator promoter feature/promoter sequence_alteration feature/mutation |