BBa_I716071

BBa_I716071 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716071

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Austin Day

Date created: 2007-06-26 11:00:00

Date modified: 2015-08-31 04:07:51

pTrc-(NO SCAR)-rbs lib-beta-rbs lib-alpha

Details

• Types: DnaRegion
• Roles: engineered_region; Composite

Description

pTrc-(NO SCAR)-rbs lib-beta-rbs lib-alpha

Notes

{{BerkiGEM2007-Biobrick2.0Intro}}

Source

Beta portion
pcr CA1154F/G00101 on 9145-I716054 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
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Alpha portion
Pcr CA1150F/CA1099R on I716070 (1727 bp)
Product is ADtemp5
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Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716071

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716071/1

Member of these Collections

• iGEM Parts Registry