Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vaibhavi Umesh
Date created: 2007-06-05 11:00:00
Date modified: 2015-08-31 04:07:51
HPI, katG
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_I716253_sequence (Version 1) |
Description
A basic part required for the chaperone/toxicity deviceNotes
{{BerkiGEM2007-Biobrick2.0Intro}}Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the HPI, katG open reading frame from Salmonella typhimurium LT2
PCR VU03F.katG /VU06R.katG on HPI, katG (1316 bp, gp = A)
PCR VU05F.katG/VU04R.katG on HPI, katG (924 bp, gp = B)
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PCR VU03F/VU04R on A+B (2212 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L)
Product is pBca9145-VU2
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VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc
VU04R.katG (External oligo) gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc
VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag
VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc