BBa_I716253

BBa_I716253 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716253
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vaibhavi Umesh
Date created: 2007-06-05 11:00:00
Date modified: 2015-08-31 04:07:51

HPI, katG



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_I716253_sequence (Version 1)

    Description

    A basic part required for the chaperone/toxicity device

    Notes

    {{BerkiGEM2007-Biobrick2.0Intro}}

    Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the HPI, katG open reading frame from Salmonella typhimurium LT2

    PCR VU03F.katG /VU06R.katG on HPI, katG (1316 bp, gp = A)
    PCR VU05F.katG/VU04R.katG on HPI, katG (924 bp, gp = B)
    ---------------------------------------------------
    PCR VU03F/VU04R on A+B (2212 bp, BglII/XhoI)
    Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L)
    Product is pBca9145-VU2
    ---------------------------------------------------
    VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc
    VU04R.katG (External oligo) gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc
    VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag
    VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc

    Source

    Salmonella typhimurium LT2

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    rdf-schema#seeAlso
    B4T0V5
     
    rdf-schema#seeAlso
    B4TCP6
     
    rdf-schema#seeAlso
    P17750
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716253/1
     

    Attachments

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