BBa_I716303

BBa_I716303 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716303

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: vincent parker

Date created: 2007-07-23 11:00:00

Date modified: 2015-08-31 04:07:52

otsBA

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_I716303_sequence (Version 1)

Description

The operon that codes for Trehalose as it is naturally found on the E.coli genome.

Notes

PCR otsBF/otsBIR on otsBA (485 bp, gp = A)
PCR otsBIF/otsAIR on otsBA (720 bp, gp = B)
PCR otsAR/otsAIF on otsBA (413 bp, gp = C)

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PCR otsBF/otsAR on A+B+C (2200 bp, BglII/XhoI)
Digest Pbca9145-Bca1144 (BglII/XhoI, 2054+910, L)
Product is Pbca9145- BBa I716303

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otsBF Forward BglII site anneals to otsB cgtaaAGATCTATGACAGAACCGTTAACCGA
otsBIF Removes internal BglII site GCAACGTATTACTCAGATtTGGCCACAAATGGCG
otsBIR Removes internal BglII site CGCCATTTGTGGCCAaATCTGAGTAATACGTTGC
otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG
otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG
otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC

Source

{{BerkiGEM2007-Biobrick2.0Intro}}

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716303/1

Member of these Collections

• iGEM Parts Registry