BBa_I716354

BBa_I716354 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716354
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Day
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:07:52

Biobricked Unmodified Hemoglobin Cassette in petDUET format



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_I716354_sequence (Version 1)

    Description

    Beta and alpha HbA subunits inserted into the commercial petDUET vector. The vector has each coding sequencing under separate T7 promoters.

    Notes

    {{BerkiGEM2007-Biobrick2.0Intro}}

    Source

    Pre-biobricking modifications to I716091.
    Quickchange AD0013F/R to get rid of EcoR1 site.
    Quickchange AD0014/F/R to get rid of BglII site.
    Quickchange AD0015F/R to get rid of XhoI site.

    PCR AD0016F/R on modified I716091.
    Digest Bgl2/Xho1 and insert into 9145 plasmid.
    Product is I716354

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716354/1
     

    Attachments

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