Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Day
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:07:52
Biobricked Unmodified Hemoglobin Cassette in petDUET format
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_I716354_sequence (Version 1) |
Description
Beta and alpha HbA subunits inserted into the commercial petDUET vector. The vector has each coding sequencing under separate T7 promoters.Notes
{{BerkiGEM2007-Biobrick2.0Intro}}Source
Pre-biobricking modifications to I716091.Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.
PCR AD0016F/R on modified I716091.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716354