BBa_I716356

BBa_I716356 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716356

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Austin Day

Date created: 2007-10-23 11:00:00

Date modified: 2015-08-31 04:07:52

Biobricked Mutant Hemoglobin Cassette in petDUET format

Details

• Types: DnaRegion
• Roles: CDS; Coding

Description

Mutant hemoglobins alpha and beta inserted into the commercial petDUET vector. Mutant beta has providence (Asn108Lys) and presbyterian (Lys82Asp) mutantions. Alpha subunit is made into a Di-alpha by a glycine linker.

Notes

{{BerkiGEM2007-Biobrick2.0Intro}}

Source

Pre-biobricking modifications to I716093.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716093.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716356

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716356/1

Member of these Collections

• iGEM Parts Registry