BBa_I716356

BBa_I716356 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716356
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Day
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:07:52

Biobricked Mutant Hemoglobin Cassette in petDUET format



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Description

    Mutant hemoglobins alpha and beta inserted into the commercial petDUET vector. Mutant beta has providence (Asn108Lys) and presbyterian (Lys82Asp) mutantions. Alpha subunit is made into a Di-alpha by a glycine linker.

    Notes

    {{BerkiGEM2007-Biobrick2.0Intro}}

    Source

    Pre-biobricking modifications to I716093.
    Quickchange AD0013F/R to get rid of EcoR1 site.
    Quickchange AD0014/F/R to get rid of BglII site.
    Quickchange AD0015F/R to get rid of XhoI site.

    PCR AD0016F/R on modified I716093.
    Digest Bgl2/Xho1 and insert into 9145 plasmid.
    Product is I716356

    igem#sampleStatus
    No part sequence
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716356/1
     

    Attachments

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