BBa_I716377

BBa_I716377 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716377

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Austin Day

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:07:52

Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with cytochromes

Details

• Types: DnaRegion
• Roles: engineered_region; Composite

Description

This part consists of the beta and alpha subunits of human hemoglobin A inserted into the commercial petDUET vector. The hemoglobin subunits are mutants with the following mutations: beta providence (Asn108Lys) and presbyterian (Lys82Asp) and the alpha subunit was made into a di-alpha fusion with a glycine linker between the two subunits.

The second part consists of the genes in the heme biosynthesis pathway hemA, hemB, hemC, and hemD.

The third part consists of the genes which encode for human cytochrome b5 and human cytochrome b5 reductase.

Notes

{{BerkiGEM2007-Biobrick2.0Intro}}

Source

Digest I716375 (BamHI/XhoI, 8218/9, Larger)

Digest I716019 (BglII/XhoI, 2063/1401, Smaller)

1,2,3 Product is I716377

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716377/1

Member of these Collections

• iGEM Parts Registry