lox66

BBa_I718016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I718016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eimad Shotar
Date created: 2007-10-25 11:00:00
Date modified: 2015-08-31 04:07:52

lox66



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_I718016_sequence (Version 1)

Description

lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics.

lox site recombination is catalysed by a Site specific recombinase, Cre.

lox sequences are composed of an 8 bp Core sequence surrounded by two Arms.

The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible".

Notes

No modidification was made on the lox66 sequence

Source

This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated.

Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI)

You can follow the construction process by following the links available in the Paris iGEM 2007 wiki:

http://parts.mit.edu/igem07/index.php/Paris
"freezer" section
plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I718016/1