BBa_I722004

BBa_I722004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I722004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuobo Shi
Date created: 2007-08-21 11:00:00
Date modified: 2015-08-31 04:07:53

Yiplac211(-EcoRI)



    • Details

    Types
    DnaRegion

    Roles
    Plasmid

    plasmid

    Sequences BBa_I722004_sequence (Version 1)

    Description

    It has a Ura 3 marker which can be used in yeast. Its transformer can grow on the CM media without uracil.

    Notes

    The EcoRI site of this plasmid must be knocked out because the EcoRI site on it may disturbe our experiment.

    Source

    (pUC19)(pBR322)(yeast 2-micron plasmid)

    Sequence Annotation Location Component / Role(s)
    M13/PUC sequencing primer
    M13/PUC reverse sequencing primer
    URA3
    AMP
    EcoRI
    		318,334
    204,220
    785,1585
    2004,2861
    285,290
    		primer_binding_site feature/primer_binding
    primer_binding_site feature/primer_binding
    feature/cds CDS
    CDS feature/cds
    point_mutation feature/s_mutation

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I722004/1
     

    Attachments

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