BBa_I724005
BBa_I724005 Version 1 Component
This part has been discontinued.
Please consider using BBa_I724006 as a replacement.
Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Susan Westfall
Date created: 2007-10-25 11:00:00
Date modified: 2015-08-31 04:07:54
Elowitz repressilator with added degradation tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I724005_sequence (Version 1) |
Description
Here is simply the standard Elowitz gene with a degradation tag on the reporter.Notes
Usage of BsrGI which cleaved the reporter in both the I5611 and E0434 thus putting the two pieces together.Source
Due to the anolmalous gel results for the I5610 digestion, a new construct of the I5610 was made using I5611 (elowitz repressilator minus the LVA) and the LVA from E0434| Sequence Annotation | Location | Component / Role(s) |
| TetR 1 BBa_R0040 -35 TetR 2 -10 conserved BBa_B0034 cI lambda LVA BBa_C0051 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop OR2 BBa_R0051 -35 OR1 -10 conserved BBa_B0034 lacI-LVA BBa_C0012 LVA barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_R0011 lac O1 -35 lac O1 -10 conserved BBa_B0034 tetR SsrA BBa_C0040 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop TetR 1 BBa_R0040 -35 TetR 2 -10 conserved BBa_B0034 AAV stop BBa_E0034 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,19 1,54 20,25 26,44 43,48 67,70 63,74 84,791 792,824 81,830 831,855 864,943 875,918 952,992 959,978 979,992 985,985 1001,1017 1001,1049 1015,1020 1025,1041 1038,1043 1062,1065 1058,1069 1076,2203 1076,2203 2165,2203 2204,2228 2237,2316 2248,2291 2325,2365 2332,2351 2352,2365 2358,2358 2374,2427 2376,2392 2393,2398 2399,2415 2416,2421 2441,2444 2437,2448 2458,3074 3075,3108 2455,3114 3115,3139 3148,3227 3159,3202 3236,3276 3243,3262 3263,3276 3269,3269 3285,3303 3285,3338 3304,3309 3310,3328 3327,3332 3351,3354 3347,3358 4082,4120 4121,4126 3365,4126 4135,4214 4146,4189 4223,4263 4230,4249 4250,4263 4256,4256 | non_covalent_binding_site feature/binding feature/BioBrick engineered_region promoter feature/promoter feature/binding non_covalent_binding_site promoter feature/promoter conserved_region feature/conserved engineered_region feature/BioBrick CDS feature/cds feature/tag tag feature/BioBrick engineered_region feature/BioBrick engineered_region engineered_region feature/BioBrick feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon feature/binding non_covalent_binding_site feature/BioBrick engineered_region feature/promoter promoter non_covalent_binding_site feature/binding promoter feature/promoter conserved_region feature/conserved engineered_region feature/BioBrick CDS feature/cds engineered_region feature/BioBrick tag feature/tag engineered_region feature/BioBrick feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon engineered_region feature/BioBrick feature/operator operator promoter feature/promoter operator feature/operator promoter feature/promoter conserved_region feature/conserved feature/BioBrick engineered_region feature/cds CDS tag feature/tag engineered_region feature/BioBrick feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon feature/binding non_covalent_binding_site engineered_region feature/BioBrick promoter feature/promoter feature/binding non_covalent_binding_site promoter feature/promoter feature/conserved conserved_region feature/BioBrick engineered_region feature/tag tag feature/stop stop_codon feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon |