| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_I725016_sequence (Version 1)
|
Description
Thiolase from C. acetobutylicum. This enzyme catalyzes the conversion of acetyl coa to acetoacetyl coa in a preliminary step prior to butanol biosythesis. The sequence has been chosen in order to provide the intial reactants for the butanol synthesis pathway at highter concentrations.
Notes
This sequence has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has a C terminal His tag. It has had its stop codon mutated to TAA.
Source
C. acetobutylicum genom. CAC_2873
