Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bernice Huang
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:07:55
MerRT: Mercury-Inducible Promoter+RBS (MerR + part of MerT)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I728456_sequence (Version 1) |
Description
This part is taken from the Mer operon on Tn501 and includes MerR, a mercury-inducible promoter, its Shine-Delgarno sequence, and the first few amino acids of MerT. MerR is a protein that binds to the Mer operon and undergoes a conformational change in the presence of Hg(II) ions which allows RNA polymerase to transcribe MerT and other proteins further downstream.Notes
The MerR protein sequence is transcribed in the reverse direction from the rest of the operon.Source
This part was PCR'ed off of plasmid pT040 from the paper "Metalloregulatory DNA-Binding Protein Encoded by the merR Gene: Isolation and Characterization" by Thomas O'Halloran and Christopher Walsh. The plasmid pT040 contains part of Tn501, Genbank accession: Z00027.| Sequence Annotation | Location | Component / Role(s) |
| MerR -35 and -10 hexamers MerT (first part) | 54,479 480,560 561,628 | feature/protein CDS feature/promoter promoter feature/cds CDS |