Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhan Jian
Date created: 2007-10-03 11:00:00
Date modified: 2015-08-31 04:07:58
Promoter Family Member (U097NUL+D062NUL)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_I732400_sequence (Version 1) |
Description
P_template1 serves as the truss for PCR cloning. By respectively applying 7 different primers upstream and downstream, that is, 49 pairs of primers in total, we have got 49 different promoters. Then we made them digsted and loaded into a vector that has already possessed a lacZ-alpha fragment. In this way, we can read from the color of the clones on the plate whether the inserted promoter works or not.By this means, We can try seven different positions for Operator A and seven for Operator B, thus constructing 7*7 kinds of promoters which have actually constituted a specific promoter family. After transformed into the four test plasmids (pCR00, pCR01, pCR10, pCR11) together with a downstream double-reporter system, we will get to know the solo-repression and co-repression effects of the two repressors on the specific promoter.