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BBa_I732702

BBa_I732702 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I732702

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhan Jian

Date created: 2007-10-13 11:00:00

Date modified: 2015-08-31 04:07:58

P_NOT_D001O22 + Double Reporters

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Details

Types	DnaRegion
Roles	engineered_region Measurement
Sequences	BBa_I732702_sequence (Version 1)

Description

P_NOT_D001O22 inserted into the promoter adapter of DPv2F (pSB3K5-I732096) using XbaI and BamHI. This plasmid is constructed for repressor screening and repression assay. The activity of P_NOT_D001O22 can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

Notes

N/A

Source

P_NOT_D001O22 inserted into the promoter adapter of DPv2F (pSB3K5-I732096) using XbaI and BamHI

Components

Access	Instance	Definition
public public public public public public public	BBa_I732202 BBa_B0034 BBa_I732006 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	Pnot(O22) BBa_B0034 lacZ-alpha BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I732202 BBa_B0034 BBa_I732006 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,124 133,144 151,384 393,404 411,1130 1139,1218 1227,1267	Pnot(O22) BBa_B0034 lacZ-alpha BBa_B0034 GFP BBa_B0010 BBa_B0012