BBa_I732709

BBa_I732709 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I732709

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhan Jian

Date created: 2007-10-13 11:00:00

Date modified: 2015-08-31 04:07:58

P_NOT_D001O16 + Double Reporters

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_I732709_sequence (Version 1)

Description

P_NOT_D001O16 inserted into the promoter adapter of DPv2F (pSB3K5-I732096) using XbaI and BamHI. This plasmid is constructed for repression assay. The activity of P_NOT_D001O16 can be quantificationally measured using ONPG assay or fluorescent assay.

Notes

N/A

Source

P_NOT_D001O16 inserted into the promoter adapter of DPv2F (pSB3K5-I732096) using XbaI and BamHI

Components

Access	Instance	Definition
public	BBa_I732271	Pnot(O16)
public	BBa_B0034	BBa_B0034
public	BBa_I732006	lacZ-alpha
public	BBa_B0034	BBa_B0034
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I732271	1,124	Pnot(O16)
BBa_B0034	133,144	BBa_B0034
BBa_I732006	151,384	lacZ-alpha
BBa_B0034	393,404	BBa_B0034
BBa_E0040	411,1130	GFP
BBa_B0010	1139,1218	BBa_B0010
BBa_B0012	1227,1267	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I732709/1

Member of these Collections

• iGEM Parts Registry