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BBa_I732722

BBa_I732722 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I732722

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhan Jian

Date created: 2007-10-13 11:00:00

Date modified: 2015-08-31 04:07:59

P_NAND_U073O22D059O46 + Double Reporters

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Details

Types	DnaRegion
Roles	engineered_region Measurement
Sequences	BBa_I732722_sequence (Version 1)

Description

P_NAND_U073O22D059O46 inserted into the promoter adapter of DPv2F (pSB3K5-I732096) using XbaI and BamHI. This plasmid is constructed for repression assay. The activity of P_NAND_U073O22D059O46 can be quantificationally measured using ONPG assay or fluorescent assay.

Notes

N/A

Source

N/A

Components

Access	Instance	Definition
public public public public public public public	BBa_I732305 BBa_B0034 BBa_I732006 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	NAND59 BBa_B0034 lacZ-alpha BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I732305 BBa_B0034 BBa_I732006 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,178 187,198 205,438 447,458 465,1184 1193,1272 1281,1321	NAND59 BBa_B0034 lacZ-alpha BBa_B0034 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_I732722/1