BBa_I732950

BBa_I732950 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I732950
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhan Jian
Date created: 2007-10-25 11:00:00
Date modified: 2015-08-31 04:08:00

P_template1 + lacZ



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences BBa_I732950_sequence (Version 1)

Description

Intermediate in the construction of P_template1

Notes

N/A

Source

N/A

Sequence Annotation Location Component / Role(s)
pBR322 origin
kanamycin resistance marker
beta-lactamase ampicillin resistance marker
beta-lactamase (ampR) promoter
BioBrick suffix
EcoRI
XbaI
Verification forward (VF2) primer binding site
Verification reverse (VR) primer binding site
E. coli his operon terminator
Deletion of AC
Stem loop
Stem loop
End of terminator
Ptemplate1?
261,880
1097,1912
2067,2927
2969,2997
1,21
3168,3173
3333,3338
3050,3069
157,176
22,93
34,35
30,64
3139,3158
3126,3129
3174,3332
sequence_feature feature/dna
feature/cds CDS
feature/cds CDS
promoter feature/promoter
sequence_feature feature/dna
sequence_feature feature/dna
sequence_feature feature/dna
primer_binding_site feature/primer_binding
primer_binding_site feature/primer_binding
feature/stem_loop stem_loop
feature/mutation sequence_alteration
stem_loop feature/stem_loop
stem_loop feature/stem_loop
stem_loop feature/stem_loop
promoter feature/promoter
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I732950/1