BBa_I732952

BBa_I732952 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I732952

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhan Jian

Date created: 2007-10-25 11:00:00

Date modified: 2015-08-31 04:08:00

Ptemplate1 + RBS.ARL2A0103

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_I732952_sequence (Version 1)

Description

{| border="1"
|-
|align="center"|Genotype
|align="center"|Character
|align="center"|Name
|-
|Top10/pT-TERM
|So not express any repressors
|align="center"|CR00
|-
|Top10/pT-ARL4A0604
|Constitutively express ARL4A0604
|align="center"|CR01
|-
|Top10/pT-ARL2A0203
|Constitutively express ARL2A0203
|align="center"|CR10
|-
|Top10/pTet-ARL4A0604-ARL2A203
|Constitutively express ARL4A0604 and ARL2A0203
|align="center"|CR11
|}

Notes

N/A

Source

N/A

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pBR322 origin	261,880	feature/dna, sequence_feature
beta-lactamase (ampicillin resistance marker)	1035,1895	CDS, feature/cds
beta-lactamase (ampR) promoter	1937,1965	promoter, feature/promoter
EcoRI	2136,2141	feature/dna, sequence_feature
BioBrick suffix	1,21	sequence_feature, feature/dna
XbaI	2301,2306	sequence_feature, feature/dna
Verification forward (VF2) primer binding site	2018,2037	primer_binding_site, feature/primer_binding
Stem loop	2107,2126	feature/stem_loop, stem_loop
End of terminator	2094,2097	feature/stem_loop, stem_loop
E. coli his operon terminator	22,93	feature/stem_loop, stem_loop
Stem loop	30,64	feature/stem_loop, stem_loop
Deletion of AC	34,35	sequence_alteration, feature/mutation
Verification reverse (VR) primer binding site	157,176	primer_binding_site, feature/primer_binding
Ptemplate1?	2142,2300	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I732952/1

Member of these Collections

• //plasmid
• //plasmidbackbone/libraryscreening/rbscodingsequence
• iGEM Parts Registry
• igem_plasmid_vectors