Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dave Curran
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:01
Light->Sustained GFP
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_I737010_sequence (Version 1) |
Description
This is the logic circuit of the 2007 University of Calgary project, and so requires the light sensor (M30109) to function. After addition of AHL to the media, the cells will be responsive to 650 nm light. When they sense light, they will begin expressing GFP (E0040), and will continue to make GFP until and AHL in the media breaks down. In the absence of either AHL or light, the bacteria should do nothing.Notes
The protein LuxR is present in the system twice; it should be expressed first upon exposure to light, and should then activate expression of yet more LuxR. This allows continued expression of GFP even when the light is removed.Source
This circuit was put together using existing biobrick parts.| Access | Instance | Definition |
| public public public public | BBa_R0084 BBa_A340620 BBa_S01414 BBa_I13504 | OmpR BBa_A340620 BBa_S01414 BBa_I13504 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0084 BBa_A340620 BBa_S01414 BBa_I13504 | 1,108 117,1115 1124,1922 1931,2805 | OmpR BBa_A340620 BBa_S01414 BBa_I13504 |