Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vladislav Lavrovsky
Date created: 2007-10-25 11:00:00
Date modified: 2015-08-31 04:08:01
PoPS->Agarase (Digests agar)
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_I737020_sequence (Version 1) |
Description
This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. Currently the gene has a EcorRI site which limits how it can be assembled.Notes
There is a EcoRI site in the sequence which must be mutated at some point.Source
Cloned out of Pseudoalteromonas atlantica T6C from the ATCC.| Sequence Annotation | Location | Component / Role(s) |
| Agarase Forward Primer Reverse Primer Start Stop | 53,2495 1,20 2571,2591 53,56 2493,2495 | CDS feature/protein primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/start start_codon stop_codon feature/stop |