Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xiaonan Wang
Date created: 2007-08-07 11:00:00
Date modified: 2015-08-31 04:08:02
dif site with forward orientation
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_I742101_sequence (Version 1) |
Description
'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct repeats of the dif site are vital to DNA monomerisation during bacterial septation. As with other recombinase sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enables recombination that is temporally isolated to septation.Notes
Constructed as two complimentary oligonucleotides.Source
E. coli K12| Sequence Annotation | Location | Component / Role(s) |
| SacI dif site (forward) | 1,3 4,31 | sequence_feature feature/misc sequence_feature feature/misc |