Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xiaonan Wang
Date created: 2007-08-07 11:00:00
Date modified: 2015-08-31 04:08:02
dif site with reverse orientation
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_I742102_sequence (Version 1) |
Description
'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct dif repeats are vital to DNA monomerisation during bacterial septation. As with other recombination sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enable recombination that is temporally isolated to division.Notes
Synthesised using two oligonucleotides.Source
E. coli K12| Sequence Annotation | Location | Component / Role(s) |
| SacI dif site (reverse) | 1,3 4,31 | feature/misc sequence_feature feature/misc sequence_feature |