Plac(rev)

BBa_I742124 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I742124

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caroline Dahl

Date created: 2007-10-21 11:00:00

Date modified: 2015-08-31 04:08:02

Reverse complement Lac promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_I742124_sequence (Version 1)

Description

This is an 'upside-down' version of I14032. It is a strong, constitual promoter. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional reglatory possibility that this gives.

The upstream sequence is non-functional and simply there to faciliate gel analysis or to physically enable the part to be recomineered (see the Edinburgh 2007 project where it is flanked by dif-sites).

Notes

When dealing with reverse complements of a locus, suffix and prefix need be fitted in opposite order.

Source

This sequence is part of the E.coli K12 genome.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CAP binding site	78,115	operator, feature/operator
LacI binding site	11,31	feature/operator, operator
Plac -35	61,66	feature/promoter, promoter
Plac -10	38,43	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_I742124/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/reverse
• //promoter
• //regulation/multiple
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry