Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03
CrtIB genes with PstI site mutated out
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_I742133_sequence (Version 1) |
Description
CrtIB encodes two enzymes, which make up steps three and two in the carotenoid biosynthesis pathway respectively.CrtI encodes phytoene desaturase and CrtB encodes Phytoene synthase.
Both cloned genes still have their native ribosome binding sites.
More information about the carotenoid synthesis pathway may be found on the Edinburgh Yoghurt Wiki
Notes
The CrtI gene contained two PstI sites, which were both mutated out using point mutagenesisSource
Genes were both isolated from Pantoea ananatis (formerly Erwinia uredovora)DSMZ 30080, ATCC 19321
Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005)
Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
| Sequence Annotation | Location | Component / Role(s) |
| native rbs CrtI coding region CrtB coding region native rbs CrtI start CrtB start CrtI stop CrtB stop | 1,22 23,1481 1498,2430 1482,1497 23,25 1498,1500 1479,1481 2428,2430 | feature/rbs ribosome_entry_site CDS feature/cds feature/cds CDS ribosome_entry_site feature/rbs start_codon feature/start start_codon feature/start stop_codon feature/stop feature/stop stop_codon |