BBa_I742135

BBa_I742135 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I742135
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caroline Dahl
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03

Natural RBS + Recombinase XerC of the XerCD complex.



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_I742135_sequence (Version 1)

    Description

    Xer C is activated by a septum-translocated FtsK at cell division. It pairs with XerD to make an activated complex which cleaves or inverts sequences at dif-sites, depending on dif-site directionality. See Edinburgh project 2007 for details.

    It turns out that XerC overexpression is a very effective way of not getting any colonies on your agar-plates. Therefore the Edinburgh 2007 team never incorperated it into its final construct.

    Notes

    The coding XerC sequence contains none of the restriction sites used when biobricking. -13bp upstream sequence included for RBS reasons.

    Source

    XerC is an innate E. coli K12 locus.

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I742135/1
     

    Attachments

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