Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:03
sam5 (coumarate hydroxylase) coding sequence
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_I742141_sequence (Version 1) |
Description
This is the coding sequence of sam5, encoding 4-coumarate-3-hydroxylase from the bacterium Saccharothrix espanaensis. The enzyme converts p-coumaric acid to caffeic acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note: contains a PstI site. This will be removed by site directed mutagenesis in due course but is still present, near the 3' end, in this version.Notes
Note that this version of the gene contains a PstI site near the 5' end. This will ultimately be removed by site directed mutagenesis.Source
Saccharothrix espanaensis DSM 44229, sequence accession ABC88666.| Sequence Annotation | Location | Component / Role(s) |
| PstI site to be removed | 1420,1425 | sequence_feature feature/misc |