Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:03
sam8+sam5 (tyrosine ammonia lyase + coumarate hydroxylase)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I742143_sequence (Version 1) |
Description
This part consists of the genes sam8 and sam5, encoding tyrosine ammonia lyase and 4-coumarate-3-hydroxylase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzymes acting together are expected to convert tyrosine to caffeic acid. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note that there is a PstI site near the 3' end of the sam5 gene in this version. This will be removed by site directed mutagenesis in due course. This construct was prepared to test activity before undertaking the mutagenesis.Notes
Note that this version of sam5 contains a PstI site near the 3' end. Thus this biobrick was prepared by inserting sam8 (EcoRI-SpeI) upstream of sam5 (EcoRI-XbaI) to avoid cutting with PstI.Source
Both genes are derived from Saccharothrix espanaensis DSM 4429.| Access | Instance | Definition |
| public public | BBa_I742148 BBa_I742147 | rbs+sam8 rbs+sam5 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I742148 BBa_I742147 | 1,1550 1559,3116 | rbs+sam8 rbs+sam5 |