Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:03
Plac+lacZ+sam8 (tyrosine ammonia lyase)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_I742144_sequence (Version 1) |
Description
This part consists of sam8, encoding tyrosine ammonia lyase (converts tyrosine to p-coumaric acid) from the bacterium Saccharothrix espanaensis DSM 44229, inserted downstream of a lac promoter with attached lacZ' minigene. The purpose of this was to ensure sam8 expression on induction with IPTG and also to provide a visually selectable tag when assembling the entire pathway; by always inserting a lacZ'-containing part into a non-lacZ'-containing part it is easy to select the correct clones on the basis that they form blue colonies.Notes
No important considerations.Source
sam8 is derived from Saccharothix espanaensis DSM 44229. The lac promoter and lacZ' minigene, encoding the N-terminal 76 amino acid residues of LacZ, are derived from Escherichia coli BS(DE3).| Access | Instance | Definition |
| public public | BBa_J33207 BBa_I742148 | BBa_J33207 rbs+sam8 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_I742148 | 1,600 609,2158 | BBa_J33207 rbs+sam8 |