Plac-YFP

BBa_I742162 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I742162

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caroline Dahl and Xiaonan Wang

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:08:03

YFP with lac promoter and lacZ

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_I742162_sequence (Version 1)

Description

This part consists of yellow fluorescent protein (E0430) preceded by a lac promoter and lacZ' minigene encoding the N-terminal part of LacZ (beta-galactosidase) for blue-white selection.

Notes

No special considerations.

Source

The lac promoter and lacZ are derived from Escherichia coli. The YFP is from the Registry.

Components

Access	Instance	Definition
public	BBa_J33207	BBa_J33207
public	BBa_B0034	BBa_B0034
public	BBa_E0030	eyfp
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J33207	1,600	BBa_J33207
BBa_B0034	609,620	BBa_B0034
BBa_E0030	627,1349	eyfp
BBa_B0010	1358,1437	BBa_B0010
BBa_B0012	1446,1486	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I742162/1

Member of these Collections

• iGEM Parts Registry