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Plac-YFP

BBa_I742162 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I742162

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caroline Dahl and Xiaonan Wang

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:08:03

YFP with lac promoter and lacZ

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_I742162_sequence (Version 1)

Description

This part consists of yellow fluorescent protein (E0430) preceded by a lac promoter and lacZ' minigene encoding the N-terminal part of LacZ (beta-galactosidase) for blue-white selection.

Notes

No special considerations.

Source

The lac promoter and lacZ are derived from Escherichia coli. The YFP is from the Registry.

Components

Access	Instance	Definition
public public public public public	BBa_J33207 BBa_B0034 BBa_E0030 BBa_B0010 BBa_B0012	BBa_J33207 BBa_B0034 eyfp BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J33207 BBa_B0034 BBa_E0030 BBa_B0010 BBa_B0012	1,600 609,620 627,1349 1358,1437 1446,1486	BBa_J33207 BBa_B0034 eyfp BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_I742162/1