Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peter Nguyen
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:08:03
LuxN-Tsr Chimeric Receptor B
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_I744209_sequence (Version 1) |
Description
TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..345) of the quorum-sensing receptor LuxN from Vibrio harveyi fused to the C-terminal signaling domain (216..551) of the E.coli MCP receptor, Tsr. The domains are linked by an NdeI site (translation = 'HM').Source
LuxN N-terminal domain is from Vibrio harveyi. Tsr C-terminal domain is from E.coli.| Sequence Annotation | Location | Component / Role(s) |
| Start translation Stop Translation LuxN sensory domain fragment 1 Tsr signaling domain fragment 2 point mutation to remove natural NdeI | 1,3 2050,2052 1,1035 1042,2052 1170,1170 | start_codon feature/start stop_codon feature/stop sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature |