BBa_I748001

BBa_I748001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I748001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kurt Whittemore
Date created: 2007-10-25 11:00:00
Date modified: 2015-08-31 04:08:05

Putative Cyanide Nitrilase Promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_I748001_sequence (Version 1)

Description

This section of DNA contains the sequence from 300 base pairs upstream of the Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase gene to 30 base pairs downstream into the gene from Pseudomonas fluorescens PfO-1. Our hope is that this region of DNA will contain the promoter for the nitrilase gene. At the time that this part was submitted, it was not tested or sequenced yet.

Notes

We isolated this segment of DNA from the Pseudomonas fluorescens PfO-1 genome using a PCR reaction.

Source

This section of DNA comes from the Pseudomonas fluorescens PfO-1 genome (NC_007492). The GeneID for the nitrilase gene that we working with is 3715134, and the protein ID for the nitrilase gene is YP_349417.1. We took the region 300 base pairs upstream of the nitrilase gene and 10 base pairs downstream into the gene.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I748001/1