Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kenichiro Iwasaki
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:08:06
BioBrick to Tokyo Standard adapter, for SpeI, forward
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_I751410_sequence (Version 1) |
Description
This part is oligonucleotide you can buy from oligo house.Annealed with rev oligo(BBa_I751411), this part can be incorporated into the space cut by SpeI.
This makes Biobrick part into Tokyo Standard part.
Notes
*[[BBtoTSD adapter]]1. Cut Biobrick part with SpeI.
2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination)
3. Now you can cut out this part by BglII or MluI.
This makes Biobrick scar-like sequence, "actaga" in the left and "tctagt" in the right. Once oligo is ligated, you can't cut with SpeI anymore.