BBa_I756027

BBa_I756027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I756027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Lei

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:08:07

LexAopMLP-GFP-SV40PA

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_I756027_sequence (Version 1)

Description

Reporter construct for signaling circuit. GFP under the control of the lexAopMLP promoter and a SV40 polyA tail. SCFV-Epor-gp130, FcGamma, Syk, P85, CD11a, CD18-Cub, and CD18-nubG all have output of lexA-VP16 which will bind the LexAop and turn on the expression of GFP.

Notes

LexAop is a repeated sequence which results in multiple products. Isolate the largest fragment for gel isolation

Source

Mfe1, ClaI (1-12bp)
LexAopMLP (minimal promoter) from pSH1834 plasmid (13-219bp)
Asc1 (220-227bp)
Kozak box (228-233bp)
GFP from PWPI lentiviral plasmid (235-981bp)
STOP STOP (983-987bp)
Xba1 (988-993bp)
Pst1 (994-999)
SV40PA from pcDNA3.1 zeo (+) plasmid (1000-1130bp)
HIndIII (1131-1136bp)

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I756027/1

Member of these Collections

• iGEM Parts Registry