Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Omar Khan
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:08:07
Syk-dnaEc-VP16AD
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I756029_sequence (Version 1) |
Description
Syk kinase with SH2 domains coupled at the C terminus to dnaEc-VP16(activation domain). This construct was inserted into pcDNA3.1(+)Zeo using XbaI and EcoRV.Notes
A flexible GS Linker (GGGGS)x5 (or more) is required between the dnaEc and Syk. This was quite difficult to clone in with the FseI site used in the part.Source
Syk from OpenBiosystems (Clone ID: 30533300)dnaEc from dnaE gene of Synechocystis sp.(split inteins)(2624-2749bp)
VP16AD from HSV1 helper virus (Sapolsky Lab, Stanford)(2750-2887bp)