Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: 2007 NYMU_Taiwan iGEM Team
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:08
CinR, CinL and glucose controlled promotor
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I761011_sequence (Version 1) |
Description
This promotor is controlled by cinr, cinl and extrcellular glucose concentration. High extracellular glucose concentration will inhibit this promotor through the action of phosphorylated OmpR. Phosphorylated OmpR will bind the OmpR binding site of the promotor and block the movement of RNA polymerase. In contrast, the combined action of CinR and CinL will turn on the promotor. But only when the extracellular glucose level is low so OmpR dephosphorylate and dissociate from its binding site will this promotor become active. In essence, this promotor function as an AND gate, it will turn on if and only if the extracellular glucose level is low and there are some functional CinR and CinL protein inside the cell.Notes
The key to the function of the promotor is the inhibitory ability of OmpR binding site. To ensure maximum affinity with phosphorylated OmpR, we use all three OmpR binding site.s.Source
*BBa_R0078*BBa_I761001
| Access | Instance | Definition |
| public public | BBa_R0078 BBa_I761001 | cinR OmpR_BS |